Gel electrophoresis is a process through which different proteins or DNA fragments are separated so that they can be identified and studied. Today, most separations require considerable manual work and are carried out on large gels which require several hours to complete. The industrial sector needs miniaturised systems capable of automatically performing a large number of separations simultaneously, and much more quickly.
PhD students Katarina Bengtsson and Sara Nilsson from the Transport and Separations Group at Linköping University have demonstrated a significant step toward miniaturised gel electrophoresis. Their finding – recently published in the scientific journal PLoS ONE
– was achieved by developing conducting polymer materials to replace platinum electrodes that are traditionally used in gel electrophoresis systems. This advance allows the stationary metal electrodes fixed in electrophoresis equipment to be replaced. The plastic electrodes can then be included as part of a disposable cassette containing the separation gel. This eliminates cross-contamination between gels run in sequence. Other issues in electrophoresis are bubble formation and pH changes caused by water electrolysis. PhD student Per Erlandsson has previously shown that the conducting polymer materials are able to be oxidised and reduced themselves, thereby eliminating the need to electrolyse water in electrokinetic systems.
Cheaper, faster and more reliable
"One of our strategies is to find ways to use these materials, developed for the printed electronics industry, in applications other than electronics and optoelectronics. We hope that this result will accelerate the automation and miniaturisation of gel electrophoresis, which in turn can make medical diagnoses and DNA sequencing cheaper, faster, and more reliable," says Assoc. Prof. Nathaniel Robinson, leader of the research group, on their "work as a logical extension of previous studies on conducting polymer electrodes in electrokinetic systems."
The technology will be further developed by the university start-up company LunaMicro AB.
Article: Conducting Polymer Electrodes for Gel Electrophoresis by Katarina Bengtsson, Sara Nilsson and Nathaniel D. Robinson. PLoS ONE open access February 2014.
Transport and Separations Group: http://www.liu.se/ifm/surfphys/transport?l=en
Åke Hjelm 2014-03-03