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Allmän apparatur på Core Facility

I två rum på plan 10 (det som tidigare kallats KEF) finns vanliga analysinstrument för ett forskningslab såson β- och γ-räknare (rum 306) och spektrofotometrar (rum 305) och ett rum är histologirum med bl a kryostater (rum 201).

 


SPARK 10M – A microplate reader optimized for cell based researchSpark10m

The SPARK 10M microplate reader can be found on Cell biology floor 13 in room 018.

The SPARK 10M microplate reader is suitable for everyday measurements of absorbance (200-1000nm; spectral scanning), fluorescence (top and bottom readings; area scanning; spectral scanning) and luminescence, as well as advanced long-lasting experiments on cell cultures under controlled conditions.

For fluorescence, it is possible to select any given wavelength between 230-900 nm (+/-20nm bandwidth) and make spectral scanning owing to the double monochromators. It is also possible to use filter-based measurements for higher sensitivity. The following filters are available; excitation: 360, 400, 485, 560, 590 and 635nm; emission: 460, 505, 530, 580, 610 and 720nm.Time-resolved fluorescence.

 

The instrument is also equipped with:

1 dispenser (5–1,000 μl; if there is a need, it is possible to purchase and install additional dispensers in the future)
CO2 and O2 gas chamber
Temperature control unit
Humidity cassette + lid lifter

Together, these features enable long-lasting cell-based assays under controlled conditions.

 

In addition, our instrument has a cell imaging and confluence measurement module which makes it possible to analyze cell confluence in different wells. For example, this has been used in applications for wound healing. For more information on this topic, please refer to this document.

 

Apparatansvarig: Björn Ingelsson bjorn.ingelsson@liu.se


ChemiDoc MP System - Cell Biologen plan 13 rum 022

chemidocThe ChemiDoc MP system is a full-feature instrument for gel or western blot imaging. It is designed to address multiplex fluorescent western blotting, chemiluminescence detection, and general gel documentation applications.

 

The fluorescent filters makes it possible to visualize gels/blots treated with various dyes (among others):

 

Stain free gels, Coomassie blue, Silver stain, Sypro Ruby, ProQ Diamond, Cy3; Cy5; Cy5.5, Alexa 546; 467; 680, Qdots 525; 605 ; 625, Rhodamine, Fluorescein

 

Although the ChemiDoc MP system can handle stained nucleic acid gels as well, we suggest the similar machine on Cell biology floor 9 for such gel imaging experiments. 

 

 

More information can be found on the BioRad homepage.

 

The ChemiDoc MP system can be found on Cell biology floor 13 in room 022.

Apparatansvarig: Björn Ingelsson bjorn.ingelsson@liu.se

 


Nytillkommet instrument på IMT:

Optisk koherenstomograf (OCT)

OCT

Ett OCT-system levererar två- eller tredimensionella bilder av vävnaders struktur baserat på optiskt spridning, jämför ultraljud fast med ljus. Detta system använder laserljus av våglängden 1300 nm, har axial upplösning på max 5.5 µm, lateral upplösning 13 µm, maximal field of view (längd×bredd×djup) 10×10×3.5 mm. Maximal a-skan-frekvens är 76 kHz.

I klinik används OCT främst inom ögon- och i viss mån kärlundersökningar. Detta system är dock inte anpassat för dessa tillämningar, utan för att i stället undersöka andra vävnader som till exempel hud.

 

Pekfinger ascanDet går också att använda för att skanna andra typer av material, förutom vävnader.

 

Till höger syns en bild på systemet samt bilder tagna på pekfinger. Hudlagrens struktur syns tydligt såväl som svettkörtlarna.

 

 

 

 

 

 

Pekfinger 3DKontakta Neda Haj-Hosseini eller Ingemar Fredriksson för introduktion.

Kostnad:
Introduktion/handledning: 650 kr/timme

Bokning av apparatur: https://www.ida.liu.se/local/mrbs/week.php?&dept=73

 

 

 

 


 

 

Histologirum med kryostater för fryssnitt, rum 201


These are precision instruments and removing ALL sectioned material from the interior of the instrument is a must for continued precise sectioning.
Always close the glass top when you leave the room. All razors and scapels are to be deposited in the special canisters found on the top of the instrument. Gloves and papers are to be thrown in the wastepaper baskets and unwanted slides disposed of in the yellow waste cartongs. Remove your specimens from the holders, wash and dry the holders and place them back into their stands within the cryocabinet. These holders are not to be taken from the labWhen reserving a cyrostat, always include your telephone number so that we/your colleges can get in touch in case the instrument should breakdown.
We recommend that you buy your own antiroll plate from Leica (art number 14047742492) and OCT compound, the “glue” that you use to fasten your specimens with.

Kryostatansvarig: Florence Sjögren, tel 010-1037718


Leica CM 1950

 Kryostat

Manual 

Boka instrumentet 

 

 

 

 

 

 

 

Leica CM 3050

Kryostat

Manual 

Boka instrumentet 

 

 

 

 

 

 

 

Microm HM 500M
Kryostat

Boka instrumentet

 

 

 

 

 

 

 

 


 

Apparatrum för analysinstrument, rum 305


Plattläsare VICTOR3
Victor

Detta är en flexibel plattläsare för absorbans, fluoroscens samt lumniscens.

Mer info om instrumentet.

Boka instrumentet

Instrumentansvarig: Elena Vikström, tel 010-103 20 54

  

 

 

 

 

Plattläsare VERSAmax för Elisa
Elisa

 

Mer info om instrumentet 

 

Boka instrumentet 

 

Kontakt: Elena Vikström, 010-103 20 54

 

 

 

 

 

BIOREADER 5000PRO-F för Elispot

 Elispot

Instrumentansvariga: Linda Åkerman tel 010-1039538 eller
Mari-Anne Åkeson, tel 010-1033764

 

Boka instrumentet


Sidansvarig: mikael.pihl@liu.se
Senast uppdaterad: Thu Nov 17 11:42:05 CET 2016