Biochemistry 2, 6 credits (TFKE36)

Biokemi 2, 6 hp

Main field of study

Biotechnology Chemical Biology Chemistry

Level

First cycle

Course type

Programme course

Examiner

Magdalena Svensson

Director of studies or equivalent

Magdalena Svensson
Course offered for Semester Period Timetable module Language Campus VOF
6CKEB Chemical Biology, M Sc in Engineering 4 (Spring 2017) 2 1+4 Swedish Linköping o
6CTBI Engineering Biology, M Sc in Engineering 4 (Spring 2017) 2 1+2 Swedish Linköping o
6IKEA Chemical Analysis Engineering, B Sc in Engineering 4 (Spring 2017) 2 1+4 Swedish Linköping v
6KBIO Biology 4 (Spring 2017) 2 1+4 Swedish Linköping v
6KKEB Chemical Biology 4 (Spring 2017) 2 1+4 Swedish Linköping o
6KKEM Chemistry 4 (Spring 2017) 2 1+4 Swedish Linköping o

Main field of study

Biotechnology, Chemical Biology, Chemistry

Course level

First cycle

Advancement level

G2X

Course offered for

  • Chemical Biology, M Sc in Engineering
  • Engineering Biology, M Sc in Engineering
  • Chemical Analysis Engineering, B Sc in Engineering
  • Biology
  • Chemical Biology
  • Chemistry

Entry requirements

Note: Admission requirements for non-programme students usually also include admission requirements for the programme and threshold requirements for progression within the programme, or corresponding.

Prerequisites

General chemistry, Organic chemsitry 1 and Biochemistry 1

Intended learning outcomes

The objective of the couse is to give comprehensive knowledge within the following areas: Enzyme kinetics, enzyme mechanisms and methodology for characterization of enzymes.After studies well learned the student will have proficiency to:

  • Calculate kinetic parameters like kcat, KM, Vmax and KI.
  • Interprete the meaning of kinetic parameters in terms of enzyme function.
  • Recapitulate the reaction mechanism for some enzymes.
  • Explore fundamental laboratory techniques and interprete the obtained data in a written report.

Course content

Theory: Structure and function of enzymes. Protein folding, enzyme kinetics with different types of inhibitions. Factors causing enzyme catalysis, characterization of the active site, examples of the reation mechansims of certain enzymes. Laborations: Purification of a protein with ionic change chromatography. Functional analysis of enzymes: Enzyme activity measurement, pH-dependance, chromatogram, kinetic- and spectrophotometric studies.

Teaching and working methods

The theory is mainly presented in lectures. Solving of problems, discussions of theoretical and practical aspects of experiments in the laboratory are performed in lessons. Theoretical and practical aspects of biochemistry are illustrated in the laboratory course. The results of the experimental work should be presented and discussed in written reports.

Examination

TEN1Written examinationU, 3, 4, 54 credits
LAB1Laboratory workU, G2 credits

The exercises of the written examination and the test elucidate how well the student perform the demands of the course.

Grades

Four-grade scale, LiU, U, 3, 4, 5

Other information

Supplementary courses: Protein Chemistry

Department

Inst för fysik, kemi och biolog

Director of Studies or equivalent

Magdalena Svensson

Examiner

Magdalena Svensson

Education components

Preliminary scheduled hours: 46 h
Recommended self-study hours: 114 h
There is no course literature available for this course.
TEN1 Written examination U, 3, 4, 5 4 credits
LAB1 Laboratory work U, G 2 credits

The exercises of the written examination and the test elucidate how well the student perform the demands of the course.

Regulations (apply to LiU in its entirety)

The university is a government agency whose operations are regulated by legislation and ordinances, which include the Higher Education Act and the Higher Education Ordinance. In addition to legislation and ordinances, operations are subject to several policy documents. The Linköping University rule book collects currently valid decisions of a regulatory nature taken by the university board, the vice-chancellor and faculty/department boards.

LiU’s rule book for education at first-cycle and second-cycle levels is available at http://styrdokument.liu.se/Regelsamling/Innehall/Utbildning_pa_grund-_och_avancerad_niva. 

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