Gene Technology, 3 credits (TFKE38)

Genteknik, 3 hp

Main field of study

Biotechnology Chemical Biology

Level

First cycle

Course type

Programme course

Examiner

Lars-Göran Mårtensson

Director of studies or equivalent

Magdalena Svensson

Available for exchange students

Yes
Course offered for Semester Period Timetable module Language Campus VOF
6MPRO Protein Science, Master's Programme (External) 1 (Autumn 2017) 1 - English Linköping o
6CKEB Chemical Biology, M Sc in Engineering 5 (Autumn 2017) 1 - English Linköping o
6KKEB Chemical Biology 5 (Autumn 2017) 1 - English Linköping o

Main field of study

Biotechnology, Chemical Biology

Course level

First cycle

Advancement level

G2X

Course offered for

  • Protein Science, Master's Programme
  • Chemical Biology, M Sc in Engineering
  • Chemical Biology

Entry requirements

Note: Admission requirements for non-programme students usually also include admission requirements for the programme and threshold requirements for progression within the programme, or corresponding.

Prerequisites

Biochemistry

Intended learning outcomes

The course will give fundamental knowledge and experimental skills in the following areas of Molecular Biology: Site-specific mutagenesis, gene cloning and PCR After studies well learned the student will have proficiency to:

  • Understand basic laboratory techniques in molecular biology techniques to perform site-directed mutagenesis, amplification of DNA fragments using PCR and cloning of DNA fragments
  • Describe different types of vectors for cloning and expression of eukaryotic genes in a prokaryotic system
  • Theoretical knowledge of DNA sequencing
  • Theoretical and practical knowledge of techniques for purification of plasmid DNA
  • Describe the function and applications of different enzymes involved in gene cloning experiments

Course content

Purifications of DNA, manipulation of DNA, cloning of genes into E.coli vectors, control of gene expression, differences in prokaryotic and eukaryotic genes, mutation analysis, DNA-sequencing.

Teaching and working methods

The theory is mainly presented in lectures. Solving of problems, discussions of theoretical and practical aspects of experiments in the laboratory are performed in lessons. Theoretical and practical aspects are illustrated in the laboratory course. The results of the experimental work should be presented and discussed in written reports. Laboration course in site-directed mutagenesis.

Examination

TEN2Written examinationU, 3, 4, 52 credits
LAB1Laboratory workU, G1 credits

Grades

Four-grade scale, LiU, U, 3, 4, 5

Other information

Supplementary courses: Protein chemistry.

Department

Institutionen för fysik, kemi och biologi

Director of Studies or equivalent

Magdalena Svensson

Examiner

Lars-Göran Mårtensson

Education components

Preliminär schemalagd tid: 42 h
Rekommenderad självstudietid: 38 h

Course literature

T.A. Brown: Gene Cloning and DNA analysis, 6th edition.
T.A. Brown: Gene Cloning and DNA analysis, 6th edition.
TEN2 Written examination U, 3, 4, 5 2 credits
LAB1 Laboratory work U, G 1 credits

Regulations (apply to LiU in its entirety)

The university is a government agency whose operations are regulated by legislation and ordinances, which include the Higher Education Act and the Higher Education Ordinance. In addition to legislation and ordinances, operations are subject to several policy documents. The Linköping University rule book collects currently valid decisions of a regulatory nature taken by the university board, the vice-chancellor and faculty/department boards.

LiU’s rule book for education at first-cycle and second-cycle levels is available at http://styrdokument.liu.se/Regelsamling/Innehall/Utbildning_pa_grund-_och_avancerad_niva. 

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