Projects

Our research projects

1. Role of epigenetic mechanisms in alcohol use and anxiety disorders

In this project, we aim at identifying the molecular mechanisms and brain circuits that promote alcohol use disorder and anxiety disorders, with a particular focus on epigenetic mechanisms.

Comorbidity of alcohol use disorder (AUD) and anxiety disorders (ANX) is a major public health problem. The identification of shared mechanisms between these disorders holds promise for developing more effective therapeutic targets. Both AUD and ANX disorders are characterized by long-term neuroadaptations within brain areas involved in regulation of negative affect. Epigenetic mechanisms, which have emerged as important mechanisms for translating environmental stimuli into specific changes in gene expression may be key players in regulating these long-term neuroadaptations. Our research, utilizing animal models that replicate key features of AUD and ANX has demonstrated a role of the epigenetic enzyme PRDM2 in alcohol associated behaviors and fear memory consolidation. This finding suggests that dysregulation of PRDM2 may represent a common mechanism in the comorbidity of AUD-ANX. Currently, using viral vector approaches and RNA sequencing, we are investigating the brain circuits and transcriptomic changes that are regulated by this enzyme. Our aim is to understand how PRDM2 modulates alcohol and anxiety-related behaviors, contributing to a more comprehensive understanding of the interplay between these disorders.

Photo credit Anna Nilsen


2. Neural circuits and transcriptomic changes in observational fear learning

In this project, we aim at identifying the neural circuits and transcriptomic changes that promote observational fear learning.

Observing others in fear or pain is a form of psychosocial stress which in humans can lead to psychiatric disorders such as anxiety disorders. Using an animal model of observational fear, we aim at identifying the molecular mechanisms and brain circuits involved in fear learning through peer observation. In previous work, we found that decreased expression of the epigenetic enzymes Prdm2 potentiated fear memory consolidation when the animals were directly experiencing a threatful stimulus. We now aim at determining whether this mechanism generalized to observational fear learning.
In addition, we aim at identifying previously unknown neurobiological substrates of observational fear using a whole brain activity mapping approach (iDISCO) combined with optogenetic approaches to determine the functional role of the identified brain regions. This integrated approach allows us to determine the functional role of the identified brain regions, providing crucial insights into the formation of observational fear memories.

Researcher in lab.Estelle Barbier. Photo credit Anna Nilsen