Our services include standard specimen preparation like fixation, embedding, single or serial sectioning, double and negative staining, correlative light and electron microscopy, freeze substitution, immunolabeling, carbon formvar coated mesh and slot grids. Our service also includes assistance with electron microscopic observation.
Bring your samples
1st step
- Contact the responsible staff at the lab (maria.ntzouni@liu.se)
- Fill in the form
- Make a work plan
2nd step
- We provide the appropriate chemicals for the first steps (EM fixatives)
- Bring you samples
- We proceed to the EM preparation of samples
3rd step
Examples of electron microscopy techniques and methods
The electron microscopy sample preparation has been done at the Microscopy Unit of Core Facility at Linköping University by maria.ntzouni@liu.se.
Equipment of the electron microscopy lab
The electron microscopy lab consists of a transmission electron microscope (field emission gun operated at 100kV). Room temperature ultra-microtomes (thin sections of 200- 1000nm and ultrathin sections of 50-80nm).
Glass knife maker for production of glass knifes for thin sectioning. Ultrathin sectioning takes place with diamond knifes.
Carbon coater and glow discharge for preparation of stable, clean, formvar coated grids.
Plunge freezers for freezing of thick tissue slides and two freeze substitutions for embedding in acrylic resins (suitable for immunolabeling). Click image to enlarge:
