Staff are in available to help, see "Contact" below. Tuition fee for external users is 600 SEK.
Cellkärnan user group: Peter Söderkvist (chairman), Henrik Green, Mats Södersröm, Jon Jonasson, Susana Cristobal.
Instruments
3500 Genetic Analyzer
Applications:
- Sequencing
- Microsatellites
- MLPATM
- LOH
- SNP
Additional information at appliedbiosystems.com
7500 Fast Real-Time PCR system
Applications: Absolut quantification, relative quantification, allele discrimination +/- detection.
Capacity: 96-well fast PCR plates.
Fast PCR reaction can be run in 30-40 min with appropriate mastermix, or in standard mode in 2h with standard mastermix.
Equipped with 5 emission filters for: SYBR® Green I, FAM™, VIC™, JOE™, NED™, TAMRA™, ROX™, Texas Red®, Cy3™, Cy5™.
Further Information at appliedbiosystems.com
7900HT Fast Real-Time PCR System
96 fast- or 384-well plate compatibility (the TaqMan® Low Density Array).
Fast PCR option reduces run time to about 35 minutes in a standard 96-well format.
A laser scans and excites the fluorescent dyes in each of the wells.
Continuous wavelength detection from 500-660 nm allows the use of multiple fluorophores in a single reaction.
MiSeq Benchtop Sequencer from Illumina (NGS)
With MiSeq, you can:
- Multiplex up to 96 samples per run for greater efficiency.
- Get accurate bi-directional amplicon sequencing.
- Generate complete de novo assemblies.
- Produce 2 x 300 paired-end reads and up to 15 Gb of data in a single run.
Agilent Bioanalyzer 2100
Users buy their own kits.
Affymetrix Microarray
Additional Information at affymetrix.com
QIAGEN Tissue Lyser
For high-throughput disruption of a wide range of biological samples
- Fast - disruption of up to 48 samples in as little as 2-4 minutes
- Disruption is achieved through the beating and grinding effect of beads
- Cross-contamination-free – closed system prevents tube-to-tube carryover
- Reproducible – highly standardized disruption methods
- Flexible – compatible with different sample types and disruption buffers
- Integrated – part of QIAGEN’s tissue-management system from collection/stabilization to purification
You have to buy your own QIAGEN kits and Beads, suitable for use with the TissueLyser system.
Additional information at qiagen.com
epMotion 5070
epMotion 5070 is a pipetting robot that allows users to write their own programs for preparing 96-well plates for PCR. Two pipettes are available, 1-50ul and 40-1000ul. Tips are purchased by individual users separately.
