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Instruments
3500 Genetic Analyzer
8-capillary platform
Applications:
• Sanger Sequencing
• Microsatellites
Old technology, but fast and inexpensive to use with simple setup, for mutation confirmation and microsatellite/STR analysis.
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MiSeq
With MiSeq, you can:
• Multiplex up to 96 samples per run for greater efficiency.
• Get accurate bi-directional amplicon sequencing.
• Generate complete de novo assemblies.
• Produce 2 x 300 paired-end reads and up to 15 Gb of data in a single run.
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Nextseq 550
With NextSeq 550, you can perform:
• Targeted sequencing (amplicon-based, gene panel)
• Transcriptome sequencing (total RNA-Seq, mRNA-Seq, gene expression profiling)
• Arrays scanning
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Nextseq 2000
With NextSeq 200, you can perform:
• Targeted sequencing (amplicon-based, gene panel)
• Small Whole Genome Sequencing (WGS)
• Whole exome sequencing (WES)
• Transcriptome sequencing (total RNA-Seq, mRNA-Seq, small RNA-Seq, single cell RNA-Seq)
For more information:
NextSeq 1000 and NextSeq 2000 Sequencing Systems | Mid-throughput benchtop sequencing (illumina.com)
To choose between the different available sequencing platforms, We recommend using the following link: Sequencing Platform Comparison Tool (illumina.com)
7500 Fast Real-Time PCR system
Capacity: 96-well fast PCR plates. Equipped with 5 emission filters for: SYBR® Green I, FAM™, VIC™, JOE™, NED™, TAMRA™, ROX™, Texas Red®, Cy3™, Cy5™.
Applications:
• Absolut quantification
• relative quantification
• allele discrimination +/- detection.
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7900HT Fast Real-Time PCR System
96fast- or 384-well plate. Old instrument with no service agreement and no spare parts available.
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QPCR QuantStudio™ 7 Flex
The QuantStudio™ 7 Flex system for 96-well Fast, 384-well, or TaqMan® array card block.
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Digital droplet PCR-system (BioRad)
The QX200 AutoDG Droplet Digital PCR System provides absolute quantification of target DNA or RNA molecules for EvaGreen and probe-based Droplet Digital PCR (ddPCR) applications.
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PCR Thermal Cycler 2720
2720 Thermal Cycler from Applied Biosystems. 96 well format.
PCR Thermal Cycler C1000
C1000 Touch Thermal Cycler from BioRad. 96 well format with gradient possibilities
Affymetrix Microarray
The Affymetrix instrument for microarray analysis is an integrated platform containing scanner, oven, fluidics station.
The microarray platform allows whole genome gene expression and miRNA expression analysis for a wide variety of organisms (Human, Mouse, Zebrafish etc.)
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Maxwell® RSC
The Maxwell® Rapid Sample Concentrator (RSC) Instrument is a platform for automated purification of nucleic acid from a range of sample types. It can process up to 16 samples simultaneously, using prefilled cartridges and preprogrammed methods. DNA or RNA extraction in 25–60 minutes, depending upon sample type.
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QIAcube Connect
QIAcube Connect performs fully automated purification of nucleic acids and proteins in molecular biology applications. It automates over 80 QIAGEN kits with over 140 standard protocols for DNA, RNA and protein sample processing.
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QIAGEN Tissue Lyser II
The Qiagen Tissue Lyser II is used for high-throughput disruption of a wide range of biological samples (animal and plant tissues, bacteria and yeast).
You must buy your own QIAGEN kits and Beads.
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Fragment analyzer 5200
Capillary electrophoresis for detection of large fragment DNA and libraries for NGS.
Analysis kits available at Cellkärnan for:
• Standard Sensitivity NGS Fragment (1 bp – 6000 bp)
• High Sensitivity NGS Fragment (1 bp – 6000 bp)
• Standard Sensitivity Large Fragment (50 bp – 20000 bp)
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Agilent Bioanalyzer 2100
The 2100 Bioanalyzer system is an established automated electrophoresis tool for the sample quality control of DNA/RNA. Agilent RNA 6000 nano kit available at Cellkärnan.
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Quantus
Quantus is a sensitive single tube fluorometer for fluorescence-based quantitation of DNA and RNA. It allows the quantification of low-level DNA/RNA samples accurately. DNA one kit and QuantiFlour RNA system are available at Cellkärnan.
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Speed Vac
Vacuum concentrator for removing solvents and concentrating samples while maintaining sample integrity.
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epMotion 5070
epMotion 5070 is a pipetting robot from Eppendorf. Four working positions and two pipettes are available, 1-50ul and 40-1000ul. Tips are purchased individually by each user.
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