The Molecular Biology unit is part of the Core Facility. It contains equipment for nucleic acid purification, amplification, sequencing, and quality control of DNA and RNA samples.

Currently, there are multiple sequencing platforms available, including the 3500 Genetic Analyzer for Sanger sequencing, MiSeq for amplicon and de novo assembly, as well as NextSeq 550 and NextSeq 2000 for targeted, whole exome, and transcriptome sequencing (including single-cell RNA-Seq). For real-time PCR applications, there are the 7500 Fast, and QuantStudio 7 Flex systems, alongside a digital droplet PCR (ddPCR) system for absolute quantification. More specialised instruments include the Fragment Analyzer 5200, Agilent Bioanalyzer 2100, and TapeStation 4150 for nucleic acid quality control, as well as Maxwell RSC and QIAcube Connect for automated DNA/RNA extraction. A Tissue Lyser II is also available for high-throughput disruption of biological samples.

Staff are available to teach new users how to use the instruments, provide advice on experimental design and data interpretation.

Use the booking calendar below to book an appointment for molecular biology instruments.

Drop-in visit, molecular biology analysis

Come and discuss your research with us and find out how our range of nucleic acid analysis techniques can advance your project. Molecular biology is essential for quantifying and characterising DNA and RNA, enabling applications from gene expression profiling to mutation detection and next-generation sequencing (NGS).

Instruments

3500 Genetic Analyzer

8-capillary platform

Applications:

  • Sanger Sequencing
  • Microsatellites

Old technology, but fast and inexpensive to use with simple setup, for mutation confirmation and microsatellite/STR analysis.

Learn more about 3500 Genetic Analyzer

MiSeq

With MiSeq, you can:

  • Multiplex up to 96 samples per run for greater efficiency.
  • Get accurate bi-directional amplicon sequencing.
  • Generate complete de novo assemblies.
  • Produce 2 x 300 paired-end reads and up to 15 GB of data in a single run.

Learn more about MiSeq

NextSeq 550

With NextSeq 550, you can perform:

  • Targeted sequencing (amplicon-based, gene panel)
  • Transcriptome sequencing (total RNA-Seq, mRNA-Seq, gene expression profiling)
  • Arrays scanning

Learn more about NextSeq 550

NextSeq 2000

With NextSeq 200, you can perform:

  • Targeted sequencing (amplicon-based, gene panel)
  • Small Whole Genome Sequencing (WGS)
  • Whole exome sequencing (WES)
  • Transcriptome sequencing (total RNA-Seq, mRNA-Seq, small RNA-Seq, single cell RNA-Seq)

Learn more about NextSeq 1000 and NextSeq 2000 Sequencing Systems

To choose between the different available sequencing platforms, we recommend using the following link: Sequencing Platform Comparison Tool (illumina.com)

CK ONT P2i

The PromethION 2 Integrated (P2i) is a compact benchtop device for high-output nanopore sequencing. It can run up to two PromethION flow cells simultaneously, allowing for flexible and efficient sequencing. The P2i includes powerful integrated compute capabilities, featuring an NVIDIA Ampere GPU, which enables real-time data processing and basecalling.

This device can generate up to 290 gigabases of data from a single flow cell. It also features a high-resolution touchscreen display and robust storage and memory specifications, making it a versatile tool for various sequencing applications.

Learn more about PromethION 2

7500 Fast Real-Time PCR system

Capacity: 96-well fast PCR plates. Equipped with 5 emission filters for: SYBR® Green I, FAM™, VIC™, JOE™, NED™, TAMRA™, ROX™, Texas Red®, Cy3™, Cy5™.

Applications:

  • absolut quantification
  • relative quantification
  • allele discrimination +/- detection.

Learn more about 7500 Fast Real-Time PCR system

QPCR QuantStudio™ 7 Flex

The QuantStudio™ 7 Flex system for 96-well Fast, 384-well, or TaqMan® array card block. 

Learn more about QuantStudio 7 Flex system

Digital droplet PCR-system (BioRad)

The QX200 AutoDG Droplet Digital PCR System provides absolute quantification of target DNA or RNA molecules for EvaGreen and probe-based Droplet Digital PCR (ddPCR) applications.

Learn more about Digital droplet PCR-system

PCR Thermal Cycler 2720 and PCR Thermal Cycler C1000

2720 Thermal Cycler from Applied Biosystems. 96 well format.

C1000 Touch Thermal Cycler from BioRad. 96 well format with gradient possibilities

Affymetrix Microarray

The Affymetrix instrument for microarray analysis is an integrated platform containing scanner, oven, fluidics station.

The microarray platform allows whole genome gene expression and miRNA expression analysis for a wide variety of organisms (Human, Mouse, Zebrafish etc.)

Click for more Learn more about Affymetrix Microarray

Maxwell® RSC

The Maxwell® Rapid Sample Concentrator (RSC) Instrument is a platform for automated purification of nucleic acid from a range of sample types. It can process up to 16 samples simultaneously, using prefilled cartridges and preprogrammed methods. DNA or RNA extraction in 25–60 minutes, depending upon sample type.

Learn more about Maxwell RSC

QIAcube Connect

QIAcube Connect performs fully automated purification of nucleic acids and proteins in molecular biology applications. It automates over 80 QIAGEN kits with over 140 standard protocols for DNA, RNA and protein sample processing.

Learn more about QIAcube Connect

QIAGEN Tissue Lyser II

The Qiagen Tissue Lyser II is used for high-throughput disruption of a wide range of biological samples (animal and plant tissues, bacteria and yeast).

You must buy your own QIAGEN kits and Beads.

Learn more about Qiagen Tissue Lyser II

CK Bioruptor Pico sonication device

The Bioruptor® Pico is a versatile sonicator for DNA, RNA, chromatin, and protein shearing with high precision and reproducibility. It allows parallel processing with built-in cooling to protect samples.

Learn more about the Bioruptor Pico

Fragment analyzer 5200

Capillary electrophoresis for detection of large fragment DNA and libraries for NGS.

Analysis kits available at Cellkärnan for:

  • Standard Sensitivity NGS Fragment (1 bp–6,000 bp)
  • High Sensitivity NGS Fragment (1 bp–6,000 bp)
  • Standard Sensitivity Large Fragment (50 bp–20,000 bp)

Learn more about Fragment analyzer 5200

Agilent Bioanalyzer 2100

The 2100 Bioanalyzer system is an established automated electrophoresis tool for the sample quality control of DNA/RNA. Agilent RNA 6000 nano kit available at Cellkärnan.

Learn more about Agilent Bioanalyzer 2100

Quantus

Quantus is a sensitive single tube fluorometer for fluorescence-based quantitation of DNA and RNA. It allows the quantification of low-level DNA/RNA samples accurately. DNA one kit and QuantiFlour RNA system are available at Cellkärnan.

Learn more about Quantus

Speed Vac

Vacuum concentrator for removing solvents and concentrating samples while maintaining sample integrity.

Learn more about Speed Vac

TapeStation 4150

The 4150 TapeStation system is a low-throughput automated electrophoresis platform for nucleic acid sample quality control. It allows a quick, easy, and reliable analysis of DNA and RNA samples for up to 16 samples per run. D100 and HS RNA kits are available at Cellkärnan.

Learn more about TapeStation 4150

NanoDrop

NanoDrop™ One is a part of Thermo Scientific™. Quantify and qualify DNA, RNA, and protein samples in seconds with only 1–2 μL, and obtain full-spectral data before you decide to use samples in downstream applications.

Learn more about NanoDrop

Project Proposal for NGS analysis and Bioinformatics support

Please fill in the Project Proposal Form
or scan the following QR-code for NGS analysis and Bioinformatics support at Core Facility, LiU and Clinical Genomics, Sweden.

QR code that takes you the form regarding Project Proposal for NGS analysis and Bioinformatics support.

Clinical Genomics

Clinical Genomics Linköping was established in 2019 as a regional distributed node of the Diagnostic and Development platform of SciLifeLab and the section of molecular genetics at the Linköping University Core unit. CG Linköping is organized at the medical faculty and operates in close collaboration with the clinical NGS platform at the University hospital in Linköping forming the local Genomic Medicine Sweden node GMC-south east.

Read more about Clinical Genomics Linköping

Contact

Booking Calender

Core Facility