18 December 2023

Scientists, including Linda Bojmar as first author, have introduced a comprehensive protocol for the cross-platform characterization of extracellular vesicles and particles (EVPs) in both humans and mice.

The protocol aims to provide a standardized approach for analyzing EVPs, crucial for unraveling their functions and exploring biomarker potential.

Key Highlights of the Protocol

Cross-Validation Across Platforms: The protocol emphasizes the cross-validation of isolated EVPs across different platforms, ensuring robust and consistent results.

Multifaceted Analysis: Researchers can now employ various techniques for EVP analysis, including nanoparticle tracking analysis and transmission electron microscopy, allowing for the examination of EVP size, number, and morphology.

Protein Cargo Identification: The protocol introduces a novel approach to EVP protein cargo identification using liquid chromatography-tandem mass spectrometry (LC-MS/MS) coupled with bioinformatic analysis.

Validation Techniques: To verify the presence of specific EVP proteins, the protocol advocates for single-particle analysis, western blot analysis, and ExoELISA, providing a comprehensive validation framework.

The significance of this protocol lies in its ability to facilitate EVP cross-validation across different platforms, enhancing the reliability of research outcomes. By offering a standardized methodology, scientists can now delve deeper into the intricate world of extracellular vesicles and particles, paving the way for breakthroughs in understanding their roles and potential as biomarkers.

This protocol is expected to catalyze advancements in the study of EVPs, opening new avenues for therapeutic development and diagnostic applications.

Contact

Latest news from LiU

Space is not just technology, also a place for culture and ethics

In the shadow of rockets, satellites and billionaires’ space projects, a new field of research is emerging. It is about understanding space also as an arena for culture, politics and ethics.

Ahead of the COP30 climate summit: “It’s looking really bad.”

Not enough is being done, and not fast enough. That is the harsh assessment made by LiU researchers Mathias Fridahl and Maria Jernnäs ahead of this year’s major international climate summit in Brazil.

Firefighter infront of wrecked car.

Research on exoskeletons and cleaners receives SEK 16.7 million

LiU receives SEK 16.7 million from AFA Försäkring for research in the field of work environment and health. The projects concern exoskeletons, cleaning staff, part-time managers and digital work environments in health and social care.