18 December 2023

Scientists, including Linda Bojmar as first author, have introduced a comprehensive protocol for the cross-platform characterization of extracellular vesicles and particles (EVPs) in both humans and mice.

The protocol aims to provide a standardized approach for analyzing EVPs, crucial for unraveling their functions and exploring biomarker potential.

Key Highlights of the Protocol

Cross-Validation Across Platforms: The protocol emphasizes the cross-validation of isolated EVPs across different platforms, ensuring robust and consistent results.

Multifaceted Analysis: Researchers can now employ various techniques for EVP analysis, including nanoparticle tracking analysis and transmission electron microscopy, allowing for the examination of EVP size, number, and morphology.

Protein Cargo Identification: The protocol introduces a novel approach to EVP protein cargo identification using liquid chromatography-tandem mass spectrometry (LC-MS/MS) coupled with bioinformatic analysis.

Validation Techniques: To verify the presence of specific EVP proteins, the protocol advocates for single-particle analysis, western blot analysis, and ExoELISA, providing a comprehensive validation framework.

The significance of this protocol lies in its ability to facilitate EVP cross-validation across different platforms, enhancing the reliability of research outcomes. By offering a standardized methodology, scientists can now delve deeper into the intricate world of extracellular vesicles and particles, paving the way for breakthroughs in understanding their roles and potential as biomarkers.

This protocol is expected to catalyze advancements in the study of EVPs, opening new avenues for therapeutic development and diagnostic applications.

Contact

Latest news from LiU

Portrait of a woman (Seetha Murty)

Two champions for children become honorary doctors

Indian principal Seetha Murty has worked with children’s and young people’s learning throughout her professional life. American professor Cindy W. Christian has devoted her career to developing the care of abused children.

Two researchers in a lab.

A single atom layer of gold – LiU researchers create goldene

For the first time, scientists have managed to create sheets of gold only a single atom layer thick. According to the researchers from LiU this has given the gold new properties that can make it suitable for use in various applications.

Researcher (Xenofon Strakosas) by a microscope.

Ten million donation for research position in electronic medicine

The Stig Wadström Foundation is donating around SEK ten million to LiU, to fund a research position in electronic medicine. The researcher chosen for this position is Xenophon Strakosas at the Laboratory of Organic Electronics in Norrköping.